Bacteriology
Discrimination between Bacteroides, Dichelobacter, Fusobacterium, Porphyromonas and Prevotella isolated from caprine footrot by
PCR-RFLP – Short communication. Ángela Lacombe-Antoneli, Segundo Píriz, Alberto
Quesada and Santiago Vadillo ..... 197
Clinical
veterinary science
Two-dimensional echocardiographic
measurements of the left atrium in dogs with cardiac disease. Zoltán
Dudás-Györki, Boglárka Bende, Csaba Hetyey, Ferenc Manczur, Jenő Reiczigel and Károly Vörös ..... 203
M-mode and two-dimensional
echocardiographic reference values for three Hungarian dog breeds: Hungarian Vizsla, Mudi
and Hungarian Greyhound. Károly Vörös, Csaba Hetyey, Jenő Reiczigel and Gábor Nagy Czirok .....
217
Effect of
citric acid on electrocardiographic parameters of broiler chickens with pulmonary
hypertension. Hossein
Hassanpour, Abdol Karim Zamani Moghaddam and Hamed Zarei ..... 229
Immunology
Novel monoclonal antibodies recognise
guinea fowl thrombocytes. Ildikó
Bódi, Nándor Nagy, Lídia Sinka, Botond-Zoltán Igyártó and Imre Oláh ..... 239
Nutrition
Pathological changes induced by rancid feed in rats and
effects on growth and protein utilisation. Sándor György Fekete, Emese Andrásofszky
and Róbert Glávits ..... 247
Pathology and
pathophysiology
Cutaneous mast cell tumour within a lipoma in a Boxer. Csaba Jakab, Attila Marcell
Szász, Janina Kulka, Zsuzsa Schaff, Miklós Rusvai, Tibor Németh and Péter Gálfi ..... 263
Harderian gland adenocarcinoma in a Florida Red-bellied Turtle
(Pseudemys nelsoni) – Case report. János Gál, Zoltán Demeter, Elena Alina Palade, Miklós Rusvai and Csaba Géczy ..... 275
Crosstalk between coagulation and inflammation in mastitis and
metritis in dairy cows. Ryszard
Bobowiec, Joanna Wessely-Szponder and Piotr Hola ..... 283
Alterations in some blood coagulation parameters in naturally
occurring cases of canine babesiosis. Renata Barić Rafaj, Vesna Matijatko, Ivana Kią,
Nada Kučer, Tatjana ®ivičnjak, Niksa Lemo, Zdravko ®vorc, Mirna Brkljačić and Vladimir Mrljak ..... 295
Gross pathology, blood chemistry,
lipid and peroxide contents in rainbow trout (Oncorhynchus mykiss Walbaum) affected by
experimental Arcobacter
cryaerophilus infection at low water temperature.
Seyit Aydin, Hayati Gure, Hasan Cakici, Serhat Colakoglu and Recep Bircan ..... 305
Physiology
and physiological chemistry
Effects of energy restriction on thyroid hormone metabolism in
chickens. Andrea Győrffy,
Ahmed Sayed-Ahmed, Attila Zsarnovszky, Vilmos László Frenyó, Eddy Decuypere and Tibor Bartha ..... 319
Reproduction
Effects of melatonin treatment on
follicular development and oocyte quality in Chios ewes – Short communication. Theodora
Tsiligianni, Irene Valasi, Sándor Cseh, Emmanuel Vainas, Vera Faigl, Fotini Samartzi,
Thomas Papanikolaou, Eleni Dovolou and Georgios S. Amiridis ..... 331
Virology
Papillomavirus-associated
fibropapillomas of red deer (Cervus elaphus).
Károly Erdélyi, János
Gál, László Sugár, Krisztina Ursu, Petra Forgách, Levente Szeredi and Theodora Steineck ..... 337
Book reviews ..... 345
DISCRIMINATION
BETWEEN BACTEROIDES, DICHELOBACTER, FUSOBACTERIUM, PORPHYROMONAS AND PREVOTELLA ISOLATED FROM CAPRINE FOOTROT BY
PCR-RFLP – SHORT COMMUNICATION
Ángela Lacombe-Antoneli1, Segundo Píriz1, Alberto Quesada2 and Santiago Vadillo1
1Unit of Microbiology,
Department of Animal Health and 2Department of Biochemistry, Veterinary
Faculty, University of Extremadura, Avda. de la Universidad s/n, 10071 Cáceres, Spain
(Received 12 September 2007; accepted 1 October 2008)
Footrot is widely considered the most severe and
most common foot pathology in small ruminants. This study tested the ability of a
molecular typing system based on polymerase chain reaction–restriction fragment length
polymorphism (PCR-RFLP) assay of the 16S rRNA gene to discriminate between the strict
anaerobe genera most commonly isolated from footrot (Bacteroides,
Dichelobacter, Fusobacterium, Porphyromonas and Prevotella) in goats in Extremadura (Spain), with a
view to facilitating identification for diagnostic purposes and thus providing a useful
tool for future epidemiological studies. Although the electrophoretic patterns obtained
with the enzyme Tru1I were more readily
interpreted, and may thus be the best initial option, results may be confirmed by a second
enzyme (RsaI). The PCR-RFLP assay of the 16S
rRNA gene may therefore prove a useful addition to conventional biochemical identification
techniques, providing taxonomic information at genus level.
Key
words: Footrot, caprine, Gram-negative
anaerobe, discrimination, PCR-RFLP
TWO-DIMENSIONAL
ECHOCARDIOGRAPHIC MEASUREMENTS OF THE LEFT ATRIUM IN DOGS WITH CARDIAC DISEASE
Zoltán Dudás-Györki1*, Boglárka Bende1, Csaba Hetyey1,
Ferenc Manczur1,
Jenő Reiczigel2 and Károly Vörös1
1Department and Clinic
of Internal Medicine and 2Department of Biomathematics and Informatics, Faculty
of Veterinary Science, Szent István University, P.O. Box 2, H-1400 Budapest, Hungary
(Received 20 May 2007; accepted 1
October 2008)
The aim of the present
study was to verify the validity of two-dimensional echocardiographic (2DE) measurement
technique in dogs with left atrial enlargement. Thirty-one dogs with clinically and
echocardiographically proven cardiac diseases were randomly selected. The left atrial
right-to-left diameter (Lar–l)
and the diameter of the left atrium just above the mitral annulus (Laama)
as well as the ratio of left atrial to aortic diameter (La/Ao)
were measured by 2DE. The Lar–l /Laama
ratio was independent of body weight (R2 = 0.0006) and age (R2 =
0.0012), respectively. A significant linear relationship was found between Laama and La/Ao (the ratio that expresses the
severity of atrial enlargement): Laama=
1.2238 La/Ao + 1.1608 (R2 = 021; P
< 0.01). There was also a positive linear relationship between Lar–l and La/Ao:
Lar–l = 1.6876 La/Ao + 1.2648 (R2 = 0.27; P < 0.005). In
our dogs with cardiac disease, the Lar–l/Laama
ratio showed no significant relation to the La/Ao index (R2 = 0.028). Thus, Lar–l/Laama proved to be
independent of the severity of atrial enlargement. Based on the strong relationship stated
in the equation between Laama and Lar–l [Lar–l /Laama= 1.31159
(95% confidence interval)], Laama can also be used for the
assessment of left atrial size even in dogs with cardiac disease.
Key
words: Left atrium, dog, atrial enlargement, echocardiography
M-MODE
AND TWO-DIMENSIONAL ECHOCARDIOGRAPHIC REFERENCE VALUES FOR THREE HUNGARIAN DOG BREEDS:
HUNGARIAN VIZSLA, MUDI AND HUNGARIAN GREYHOUND
Károly Vörös1*, Csaba Hetyey1, Jenő Reiczigel2 and
Gábor Nagy Czirok3
1Department and Clinic
of Internal Medicine and 2Department of Biomathematics and Informatics, Faculty
of Veterinary Science, Szent István University, István u. 2, H-1078 Budapest, Hungary; 3Biovet
Állatorvosi Rendelő, Budapest, Hungary
(Received 30 May 2008; accepted 1
October 2008)
The aim of the study was
to establish normal reference echocardiographic values for three Hungarian dog breeds, and
to determine the potential dependence of intracardiac parameters on body weight, age and
gender. M-mode and two-dimensional echocardiography were performed on 95 clinically
healthy dogs including 45 Hungarian Vizslas, 28 Mudis and 22 Hungarian Greyhounds. Linear
intracardiac measurements included interventricular septal thickness (IVS), left
ventricular internal diameter (LVID), left ventricular posterior wall thickness (LVPW)
both in systole and diastole, as well as left atrial internal diameter (LAD), and aortic
diameter (AOD) in early diastole. Fractional shortening (FS), end-diastolic and
end-systolic left ventricular volumes (EDV and ESV), as well as LAD:AOD ratio were
calculated from the linear parameters. Mean, range and standard deviation of measurements
were calculated for each breed. Body weight positively correlated in all three breeds with
all left ventricular dimensions, such as IVSd, IVSs, LVIDd,
LVIDDs, LVPWd and LVPWs parameters. LA values showed
positive correlations to body weight in all three breeds. AOD and LA demonstrated a
positive correlation with body weight in Hungarian Vizslas and Mudis, whilst the LAD:AOD
ratio was related to body weight only in Mudis. Gender did not correlate with any of the
measured echocardiographic parameters in any breeds. In Mudis, a positive correlation was
found between the LAD:AOD ratio and age, as well as between the LAD:AOD ratio and E point to septal separation (EPSS).
Key
words: Echocardiography, measurement, dog,
normal values, two-dimensional, M-mode
EFFECT OF CITRIC ACID ON
ELECTROCARDIOGRAPHIC PARAMETERS OF BROILER CHICKENS WITH PULMONARY HYPERTENSION
Hossein Hassanpour1, Abdol Karim Zamani Moghaddam2 and Hamed Zarei3
1Department of Basic Sciences, 2Department
of Clinical Sciences, College of Veterinary Medicine, Shahrekord University, Shahrekord,
Iran; 3Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic
Azad University, Shahrekord Branch, Shahrekord, Iran
(Received 1 May 2008; accepted 1
October 2008)
To clarify the effect of
orally administered citric acid on the electrocardiographic parameters of pulmonary
hypertensive broilers, chicks were reared at high altitude and treated with citric acid
for 45 days. Increase of cardiac indices such as right ventricle/total ventricles (RV/TV),
RV/body weight and TV/body weight ratio was not observed in the treated groups as compared
to the control groups. Decrease of S amplitude in the treated groups (leads II, III, aVF)
was only significant (P < 0.05) at 45 days of age (leads II, aVF; 1.5 g/l of citric
acid). There were significant reductions of T amplitude at 28 (lead aVR) and 36 days (lead
aVL) and significant reductions of R wave at 28 (lead aVL, all doses of citric acid) and
45 days of age (lead aVR, 1 and 1.5 g/l citric acid) in the treated groups. The QRS
interval was significantly higher at 28 (leads III and aVF), 36 (leads II, III and aVF, 1
g/l citric acid) and 45 days of age (leads II and aVF) in the treated groups. The QT
interval was significantly (P < 0.05) increased at 28 and 45 days of age (lead aVL) and
the RR interval also showed an increase at 28 (leads aVR and aVL) and 45 (lead aVL) days
of age as compared to the controls. Our data indicate that citric acid modulates the
induction of pulmonary hypertension at high altitude and this effect is detectable in the
electrocardiographic parameters.
Key
words: Citric acid, electrocardiographic
parameters, broiler, altitude, pulmonary hypertension
NOVEL
MONOCLONAL ANTIBODIES RECOGNISE GUINEA FOWL THROMBOCYTES
Ildikó Bódi, Nándor Nagy, Lídia Sinka, Botond-Zoltán Igyártó
and Imre Oláh
Department of Human Morphology and
Developmental Biology, Faculty of Medicine, Semmelweis University, Tűzoltó u.
58, H-1094 Budapest, Hungary
(Received 22 May 2008; accepted 1
October 2008)
This paper introduces
two novel monoclonal antibodies, designated GTr1 and GTr2, which recognise guinea fowl
thrombocyte surface antigen(s). The antibodies were tested in embryos and adult birds.
GTr1 and GTr2 staining emerged at embryonic days 12 and 7, respectively. After embryonic
day 12 there was no difference in staining pattern between the two monoclonal antibodies.
The isotype of the antibodies is IgG1. The antibodies did not react with any other
haematopoietic cells of guinea fowl, and there was no species cross-reaction with chicken,
turkey and quail. The antibodies can be used in interspecies chimeric and parabiotic
experiments to identify cells of guinea fowl origin.
Key
words: Guinea fowl, avian, monoclonal
antibodies, thrombocyte
PATHOLOGICAL
CHANGES INDUCED BY RANCID FEED IN RATS AND EFFECTS ON GROWTH AND PROTEIN UTILISATION
Sándor György Fekete1, Emese Andrásofszky1 and Róbert Glávits2
1Institute of Animal
Breeding, Nutrition and Laboratory Animal Science, Faculty of Veterinary Science, Szent
István University, P.O. Box 2, H-1400 Budapest, Hungary; 2Central Agricultural
Administrative Office, Veterinary Diagnostic Management, Budapest, Hungary
(Received 4 June 2008; accepted 1
October 2008)
The objective of this
trial was to study the effect of rancid feeds on the health status and growth of rats and
to determine the pathological changes induced by
dietary rancidity. Forty-two weaned male rats (body weight: 69.3 ± 1.0 g) were divided into seven experimental groups (n = 6 each). Rats in the
different groups were fed diets containing meat-and-bone meal at an inclusion rate of 19
to 22%, low or high in peroxides and high in organic acids, with or without antioxidant.
The diets were isoproteic (10%) and isolipidic (6%). During the 26-day-long trial (5 days
for adjustment and 21 days for the main period) the body weight gain and the feed
consumption were recorded. At the end of the trial detailed gross and histopathological
examinations were performed. Feeding high-peroxide feed mixtures for 21 days significantly
(P < 0.05) decreased feed intake and liveweight gain, while high organic acid
concentration had only slight negative effects. Antioxidant supplementation alleviated the
harmful effects, especially in the high-peroxide group. The ingestion of rancid feed
mixtures and the subsequent decreased feed intake caused a decrease of glycogen content in
the hepatocytes, accompanied by a slight centrolobular fatty infiltration. Peroxides
caused lymphocyte depletion in the spleen, decreased the size of Malpighian bodies and the
number of lymphoblasts, and altered the spermatogenesis. The protective effect of the
antioxidant mixture seemed to be negligible in this respect.
Key
words: Dietary peroxides, rat, lymphocyte depletion, spermatogenesis, growth,
antioxidant
CUTANEOUS
MAST CELL TUMOUR WITHIN A LIPOMA IN A BOXER
Csaba Jakab1, Attila Marcell Szász2, Janina Kulka2, Zsuzsa Schaff2, Miklós Rusvai1, Tibor Németh4 and Péter Gálfi3
1Department of
Pathology and Forensic Veterinary Medicine, 3Department of Pharmacology and
Toxicology and 4Department of Surgery and Ophthalmology, Faculty of Veterinary
Medicine, Szent István University, István utca 2, H-1078 Budapest, Hungary; 22nd
Department of Pathology, Semmelweis University, Budapest, Hungary
(Received 30 April 2008; accepted 1
October 2008)
This report describes a
case of a canine cutaneous grade I mast cell tumour which developed within a lipoma in the
right axillar region of an 8-year-old male Boxer. Immunohistologically, the neoplastic
mast cells were positive for serotonin, CD45 vimentin and p53, and negative for lysozyme,
CD3 and CD79a expression. The proliferation index of the mast cell tumour based on the
Ki-67 antigen was 6.1%. Between the benign neoplastic lipocytes and mastocytoma tumour
cells intratumoural microvessels were detected by immunohistochemical staining using CD31
and claudin-5 as markers for vascular endothelium.
Key
words: Lipoma, mast cell tumour, tumour in tumour, canine, immunohistochemistry
HARDERIAN
GLAND ADENOCARCINOMA IN A FLORIDA RED-BELLIED TURTLE (PSEUDEMYS NELSONI) – CASE REPORT
János Gál1, Zoltán Demeter1, Elena Alina Palade1, Miklós Rusvai1 and Csaba Géczy2
1Department of
Pathology and Forensic Veterinary Medicine, Szent István University, Faculty of
Veterinary Science, István u. 2, H-1078 Budapest, Hungary; 2Dr. Graf
Veterinary Practice, Budapest, Hungary
(Received 9 June 2008; accepted 1
October 2008)
The authors describe a
case of unilateral adenocarcinoma emerging from the Harderian gland, filling the right
orbital cavity of a Florida Red-bellied Turtle (Pseudemys
nelsoni). The tumour did not produce any metastasis but presented an expansive growth
and led to the dislocation and protrusion of the right eyeball. Histopathological analysis
revealed the presence of numerous mitotic figures in the cellular population that made up
the tumour. The tumour cells completely filled the alveoli of the gland and had a
nest-like structure. The authors also emphasise the importance of the differential
diagnosis of this rare pathological change in turtles. Epithelial hyperplasia of the
Harderian gland’s duct, observed in animals suffering from vitamin A deficiency, can
also lead to an enlargement of the eyelid, but in these cases the change usually involves
both eyelids symmetrically. This is the first description of a Harderian gland
adenocarcinoma in a Florida Red-bellied Turtle.
Key
words: Harderian gland, adenocarcinoma,
Florida Red-bellied Turtle, Pseudemys nelsoni
CROSSTALK
BETWEEN COAGULATION AND INFLAMMATION IN MASTITIS AND METRITIS IN DAIRY COWS
Ryszard Bobowiec1, Joanna Wessely-Szponder1 and Piotr Hola2
1Department of
Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine,
University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland; 2Veterinary
Clinic BO-VET, Hańsk, Poland
(Received 19 May 2008; accepted 1
October 2008)
Coagulation and
inflammation are closely related as part of the mechanisms of host defence during a severe
infection. The aim of this study was to investigate the relation between thrombin as a
factor in both the coagulative and inflammatory processes and neutrophil secretory
function on the basis of lactoferrin (LF), elastase and myeloperoxidase release in the
course of mastitis and metritis in cows.
Thrombin generation was measured on the basis of hydrolysis of SAR-PRO-ARG-pNA and
lactoferrin concentration was estimated by an ELISA method. The greatest thrombin
generation was observed in the metritis group (1.18 ± 0.62 IU). The level of LF was the
highest in the group of cows with mastitis (0.74 ± 0.55 mg/ml) in the first phase of the
disease. In the second phase of the diseases the level of serum LF in cows with mastitis
diminished to the value of 0.41 ± 0.16 mg/ml, whereas in cows with metritis the level of
LF increased to 0.51 ± 0.17 mg/ml. This study reveals that the excessive production of
thrombin not only causes hypercoagulatory disorders but also exaggerates neutrophil
function by the release of some enzymes which may play a destructive role during
disseminated intravascular coagulation (DIC). These enzymes also inhibit anticoagulative
systems, thus potentially worsening the course of the disease.
Key
words: Mastitis, metritis, DIC, thrombin, coagulation, inflammation, dairy cow
ALTERATIONS IN SOME BLOOD
COAGULATION PARAMETERS IN
NATURALLY OCCURRING CASES OF CANINE BABESIOSIS+
Renata Barić Rafaj1, Vesna Matijatko2, Ivana Kią2, Nada Kučer2, Tatjana ®ivičnjak3, Niksa Lemo2, Zdravko ®vorc2, Mirna Brkljačić2 and Vladimir Mrljak2
1Department of
Chemistry and Biochemistry, 2Clinic of Internal Medicine and 3Department
of Parasitology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55,
10000 Zagreb, Croatia
(Received 30 January 2008; accepted 1
October 2008)
Changes in coagulation
parameters were studied in dogs naturally infected with Babesia canis canis (n = 30), and haemostasis was
evaluated and compared to values obtained from healthy dogs (n = 29). To date, there have
not been any studies examining the dynamics of thrombin-antithrombin complex formation in
cases of canine babesiosis. Coagulation parameters evaluated before (day 0) and on days 1,
2, and 3 after treatment with imidocarb (6 mg/kg inj. s.c.) included the determination of platelet counts, the formation of
thrombin-antithrombin complexes (TAT), prothrombin time (PT), activated partial
thromboplastin time (APTT) and antithrombin III (AT III) activity. TAT complexes were
significantly elevated in animals with babesiosis on days 0 and 2 (mean 49.7 and 87.7
µg/L vs. control, 7.2 µg/L). AT III activity was significantly decreased at all
time-points examined. There were no differences in PT. On days 2 and 3 the APTT was
significantly shortened in the infected dogs when compared to control animals (means of
21.3 and 19.2 s vs. control, 30.0 s). Our analysis demonstrated that infected dogs had
significant thrombocytopenia during the course of the study (mean day 0 – 29 × 109/L,
day 1 – 48 × 109/L, day 2 –
47 × 109/L and day 3 – 87
× 109/L, vs.
control – 259 × 109/L). These data suggest that
babesiosis in dogs compromises primary and secondary haemostasis and that induction of
disseminated intravascular coagulation (DIC) occurs in canine babesiosis.
Key
words: Dog, Babesia canis, haemostasis,
coagulation
GROSS PATHOLOGY, BLOOD CHEMISTRY,
LIPID AND PEROXIDE CONTENTS IN RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM) AFFECTED
BY EXPERIMENTAL ARCOBACTER CRYAEROPHILUS INFECTION AT LOW WATER TEMPERATURE
Seyit Aydin1, Hayati Gure2, Hasan Cakici3, Serhat Colakoglu2 and Recep Bircan4
1Education Faculty, Kastamonu University,
37200 Kastamonu, Turkey; 2Directory of Çanakkale, Ministry of Rural Affairs,
Canakkale, Turkey; 3Medicine College, Canakkale Onsekiz Mart University,
Canakkale, Turkey; 4Fisheries Faculty, Ondokuz Mayis University, Sinop, Turkey
(Received 29 April 2008; accepted 1
October 2008)
Arcobacter
cryaerophilus was isolated from naturally infected rainbow trout (Oncorhynchus
mykiss Walbaum), and its pathogenicity was tested by intramuscular injection using
healthy 1-year-old rainbow trout under cold-water conditions (at 5 °C). The lethal dosage of 50% end point (LD50) for A.
cryaerophilus was calculated as 7.79 × 105 viable cells. Experimental
infection caused gross clinical abnormalities such as fallen scales, exophthalmia, oedema
in injection region and at the base of fins, pale gills, kidney necrosis, hyperaemic areas
in pale liver, haemorrhagic spots in heart, elongated spleen and swollen gallbladder.
Activities of aspartate aminotransferase and alkaline phosphatase, and concentrations of
glucose, total protein, albumin, cholesterol, triglyceride and calcium in the serum of the
experimentally infected rainbow trout were significantly decreased compared with the
healthy fish. Positive correlations were observed among blood parameters. Total lipid
weights increased in the brain, muscle and liver tissues of infected fish and dropped in
the gill and spleen tissues. Lipid peroxide contents in the brain, liver, kidney, spleen,
muscle and gill tissues of infected rainbow trout were significantly higher than in
healthy animals. The present work shows that A. cryaerophilus can be
moderately virulent for rainbow trout at low water temperature, and changes in lipid and
lipid peroxide contents of tissues and blood indices can highlight barely detectable
effects of A. cryaerophilus infection in rainbow trout under laboratory
conditions. However, the application of these indices in farm biomonitoring using rainbow
trout will need more detailed studies and a careful consideration of the environmental
parameters.
Key
words: Arcobacter cryaerophilus, disease, pathogenicity, rainbow trout, blood,
enzyme, metabolite, electrolyte, lipid, peroxide, temperature
EFFECTS
OF ENERGY RESTRICTION ON THYROID HORMONE METABOLISM IN CHICKENS
Andrea Győrffy1, Ahmed Sayed-Ahmed2, Attila Zsarnovszky1,
Vilmos László Frenyó1, Eddy Decuypere3 and Tibor Bartha1*
1Department of
Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University,
István u. 2, H-1078 Budapest, Hungary; 2Department of Anatomy, Faculty of
Veterinary Medicine, Alexandria University, Damnhur Branch, Egypt; 3Laboratory
for Farm Animals, Catholic University of Leuven, Heverlee, Belgium
(Received 22 May 2008; accepted 1
October 2008)
Energy restriction
induces changes in thyroid hormone economy in the form of a complex adaptation mechanism,
in order to conserve energy storage and protein reserves. In the present work, thyroid
hormone serum concentrations, hepatic deiodinase enzyme activities and hepatic deiodinase
mRNA expression were examined after feed restriction and fasting. We demonstrate that
during energy restriction, T3 concentration is lowered due to a decreased T4
activation and increased T3 inactivation. We show that hepatic type-I
deiodinase (D1) is not affected by energy restriction, however, hepatic D2 is decreased on
both transcriptional and enzyme activity levels. Furthermore, hepatic D3 is increased
after feed restriction in the liver. We also show that the hypothalamic feedback is not
involved in the changes in serum T3 and T4 concentrations. Our data
indicate that D2 enzyme contributes to the special hormone-exporting role of the chicken
liver and this enzyme can be modulated by feed restriction.
Key
words: Chicken, liver, deiodination, energy restriction
Corresponding author; E-mail:
bartha.tibor@aotk.szie.hu; Fax: 0036 (1) 478-4165
EFFECTS
OF MELATONIN TREATMENT ON FOLLICULAR DEVELOPMENT AND OOCYTE QUALITY IN CHIOS EWES – SHORT COMMUNICATION
Theodora Tsiligianni1,2, Irene Valasi1, Sándor Cseh3, Emmanuel Vainas2, Vera Faigl3, Fotini Samartzi2, Thomas Papanikolaou1, Eleni Dovolou1 and Georgios S. Amiridis1
1Clinic of Obstetrics
and Reproduction, Veterinary Faculty, University of Thessaly, 224 Trikalon str., 43100
Karditsa, Greece; 2NAGREF, Thessaloniki, Greece; 3Faculty of
Veterinary Science, Szent István University, Budapest, Hungary
(Received 22 April 2008; accepted 1
October 2008)
Follicular development
and oocyte quality were assessed by laparoscopic observation and in vitro fertilisation, respectively, in
melatonin-treated (Group M) and control (Group C) anoestrous Chios ewes (n = 10 in each group). Fourteen days after melatonin
insertion, all ewes had laparoscopic evaluation of the follicular population followed by
oocyte pick-up (OPU); on day 22 intravaginal progestagen sponges were inserted for 14
days. Two days after sponge removal the follicular population was re-evaluated and a
second follicular aspiration was performed. Collected oocytes from the second OPU
underwent in vitro maturation, fertilisation and
culture. The number of large follicles was higher in Group M than in the control ewes
during the first OPU and tended to be so (P = 0.06) at the second. Morphologically,
oocytes collected from controls were of better quality than those from Group M; however,
more oocytes collected from melatonin-treated animals fertilised and developed in vitro. These results indicate that melatonin
is a potent regulator of follicular development and oocyte competence during the
anoestrous period of the ewe.
Key
words: Melatonin, oocyte, embryo, OPU, sheep
PAPILLOMAVIRUS-ASSOCIATED
FIBROPAPILLOMAS OF RED DEER (CERVUS ELAPHUS)
Károly Erdélyi1, János Gál2, László Sugár3, Krisztina Ursu1, Petra Forgách4, Levente Szeredi1 and
Theodora Steineck5
1Central Agriculture
Office – Veterinary Diagnostic Directorate, Tábornok u. 2, H-1149 Budapest, Hungary; 2Department
of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent
István University, Budapest, Hungary; 3Faculty of Animal Science, University
of Kaposvár, Kaposvár, Hungary; 4Department of Microbiology and Infectious
Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary; 5Research
Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, Austria
(Received 16 July 2008; accepted 1
October 2008)
Oval, firm, cutaneous
tumours with a rough, hairless, pigmented surface, exhibiting a moderately pronounced
papillary structure were detected on the abdominal skin of two young red deer (Cervus
elaphus). One animal was shot in Lower Austria in 2004, the other at a deer farm in Hungary in 2007. Histological examination of both samples classified the tumours as
fibropapillomas, showing marked proliferation of fibroblasts and connective tissue,
accompanied by hyperkeratosis, parakeratosis and acanthosis of the overlaying epidermis,
and occasional foci of inflammation. The distribution of cytokeratin and vimentin was
characterised in the lesion. The presence of papillomavirus (PV) antigen was demonstrated
by immunohistochemistry in both cases. Papillomavirus-specific DNA was successfully
amplified by PCR from one sample. The obtained partial nucleotide sequence of the L2 ORF
exhibited the highest critical identity values with the homologous regions of
Delta-papillomaviruses, especially the Roe deer papillomavirus (93%). Phylogenetic
analysis of the partial L2 ORF sequence alignment of 10 papillomaviruses by both
neighbour-joining and maximum parsimony method confirmed that the Red deer PV is very
closely related to the Western roe deer papillomavirus (CcPV1).
Key
words: Fibropapilloma, papillomavirus, red deer, tumour, PCR
BOOK
REVIEWS
Klaus-Dieter Budras, W. O.
Sack and Sabine Röck: Anatomy of the Horse. An Illustrated
Text. 5th, revised edition with Aaron Horowitz and Rolf Berg, Schlütersche GmbH
& Verlag und Druckerei, Hanover, Germany, 2009. 199 pages, 45 colour plates, 203
figures, 21 tables. Price: € 82. Hardcover, 24.5 × 34.3 cm. ISBN: 978-3-89993-044-3.
Péter
Sótonyi
Jörg M. Steiner (editor): Small Animal Gastroenterology. Schlütersche Verlags-gesellschaft
mbH & Co. KG, Hannover, 2008. Hardcover, 384 pages with 281 illustrations,
81 × 11. Price: € 129 / L 88 / USD 175.99. ISBN: 978-3-89993-027-6.
Ágnes
Sterczer
