Acta Veterinaria Hungarica 49 (1) (2001)
CONTENTS AND ABSTRACTS
Animal welfare
Questionnaire assessment of the welfare of pigs.
G. Horváth and L. Visnyei
1-10Bacteriology
DNA sequence of a small, unidentified plasmid isolated from a Haemophilus somnus strain. Short communication.
R. Izadpanah, Á. Dán, Mária Benkő, M. Rusvai, L. Fodor and B. Harrach
11-16Immunology
Evaluation of vaccine-induced maternal immunity against classical swine fever.
P. Soós, M. Mojzis, A. Pollner and L. Sümeghy
17-24Age- and sex-determined differences in the establishment of tetanus antitoxin production in guinea pigs.
L. A. Réthy, Magdolna Géresi and L. Réthy
25-30Pharmacology
Pharmacokinetics and bioavailability of doxycycline in fasted and nonfasted broiler chickens.
P. Laczay, G. Semjén, J. Lehel and G. Nagy
31-37Physiology
Mechanism of action of noradrenaline on secretion of progesterone and oxytocin by the bovine corpus luteum in vitro.
Grażyna Miszkiel and J. Kotwica
39-51Peripheral circulating insulin-like growth factor-I and -II in cattle.
Judith Anna Nikolić, Olgica Nedić, H. Šamanc, S. Aleksić, B. Miščević and Margit Kulcsár
53-63Insulin-like growth factor binding proteins and mitogenic activity of partially fractionated sheep amniotic fluid.
J. Blahovec, Zuzana Kostecká, Françoise Cavaille, M. G. Lacroix and J. Mester
65-70Apoptotic cell death in the porcine endometrium during the oestrous cycle.
Barbara Wąsowska, Beata Ludkiewicz, Stanisława Stefańczyk-Krzymowska, W. Grzegorzewski and Janina Skipor
71-79Absorption of leucine, alanine and lysine from the rumen.
T. Veresegyházy, Hedvig Fébel and Ágnes Rimanóczy
81-86Radiology
Radiodiagnostic examination of the swimbladder of some fish species.
A. Beregi, Cs. Székely, L. Békési, J. Szabó, V. Molnár and K. Molnár
87-98Reproduction
Preimplantation genetic diagnosis in cattle: A review.
S. Bodó, B. Baranyai, Elen Gócza, J. Dohy and Merja Markkula
99-109Surgery
The surgical technique and the age of the horse both influence the outcome of mosaicplasty in a cadaver equine stifle model.
G. Bodó, A. D. Kaposi, L. Hangody, J. Tóth, Z. Bakos, Z. Lukács and G. Péntek
111-116Virology
Pathogenicity of a bovine viral diarrhoea virus strain in pregnant sows: Short communication.
G. Kulcsár, P. Soós, L. Kucsera, R. Glávits and V. Pálfi
117-120Book reviews
121-124
Acta Veterinaria Hungarica 49 (1), pp. 1–10 (2001)
QUESTIONNAIRE ASSESSMENT OF THE WELFARE OF PIGS
G. Horváth* and L. Visnyei
Department of State Veterinary Medicine and Agricultural Economics,
Faculty of
Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary
(Received April 27, 2000; accepted October 30, 2000)
A questionnaire study was performed involving 76 farms with a total of 380,207 pigs to assess the welfare of pigs kept in Hungary based on the animal welfare legislation of the European Union. Most significant deficiencies were found in the fulfilment of the provisions relating to space requirements, the stall microclimate and the behavioural needs of the animals.
Key words: Pigs, welfare assessment, questionnaire survey, legislation
*
E-mail: ghorvath@univet.hu; Fax: +36 (1) 478-4186Acta Veterinaria Hungarica 49 (1), pp. 11–16 (2001)
DNA SEQUENCE OF A SMALL, UNIDENTIFIED PLASMID ISOLATED FROM A HAEMOPHILUS
SOMNUS STRAIN:
SHORT COMMUNICATION
R. Izadpanah1,4, Á. Dán2,3, Mária
Benkő2, M. Rusvai1, L. Fodor1* and B. Harrach21
Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, H-1581 Budapest, P.O. Box 22, Hungary; 2Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary; 3Central(Received January 31, 2000; accepted October 30, 2000)
One of the plasmids present in a Haemophilus somnus strain isolated from nasal discharge of a cattle with respiratory disease was purified and cloned for DNA sequencing. The plasmid was found to be 1065 base pairs long with 39.2% G+C content, and showed no homology to any DNA sequenced so far. It has no capacity to code any protein longer than 43 residues. It is not clear yet if this plasmid codes Haemophilus somnus specific factors.
Key words: DNA, sequencing, plasmid, cattle, Haemophilus somnus
*
Corresponding author; E-mail: fodor@novell.vmri.hu; Fax: +36 (1) 251-9260Acta Veterinaria Hungarica 49 (1), pp. 17–24 (2001)
EVALUATION OF VACCINE-INDUCED MATERNAL
IMMUNITY AGAINST CLASSICAL SWINE FEVER
1
Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, H-1581 Budapest, P.O. Box 22, Hungary; 2State Veterinary Institute, Zvolen, Slovakia; 3Agricultural State Farm, Mezőhegyes, Hungary(Received April 10, 2000; accepted May 3, 2000)
The vaccine-induced maternal immunity against classical swine fever (CSF) was investigated in this study. Eight sows were vaccinated with the Chinese strain of classical swine fever virus (CSFV). The length of time between vaccination and farrowing was 167–217 days. Milk samples from the front, middle and back udder sections and blood samples were taken from the sows on days 3 and 14 after farrowing. Blood samples were obtained from the piglets at the age of 3, 6 and 10 weeks. The antibody level of the milk was examined by ELISA, and that of blood samples by the virus neutralization (VN) test as well. In all 3-week-old piglets and in 80% of the 6-week-old animals the neutralizing antibody level reached the titre of 1:40. In none of the 10-week-old piglets did the titre exceed the value of 1:20, but in about 25% of the piglets it reached 1:20; the half of these piglets came from two litters. In none of the piglets did the antibody level reach the negative threshold in the ELISA test during the study. No significant differences were found between the udder sections in milk antibody level by ELISA.
Key words: Classical swine fever, maternal immunity, colostrum, vaccination
*
E-mail: soos@novell.vmri.hu; Fax: +36 (1) 251-9260Acta Veterinaria Hungarica 49 (1), pp. 25–30 (2001)
AGE- AND SEX-DETERMINED DIFFERENCES
IN THE ESTABLISHMENT OF TETANUS ANTITOXIN PRODUCTION IN GUINEA-PIGS
L. A. Réthy1*, Magdolna Géresi2 and L. Réthy1+
1Independent Research Group for Immunology and Genetics, H-1111
Budapest,
Egry J. u. 19–21, Hungary; 2State Serum and Vaccine Institute ‘Phylaxia’,
Budapest, Hungary
(Received April 20, 2000; accepted October 30, 2000)
For lack of relevant data of the literature, the tetanus immunisation results obtained in the two sexes were compared in an animal model. Complete immunisation series of weaned, adult and aged guinea-pigs (20–25 animals/group) were performed with aluminium phosphate (AlPO4) adsorbed purified tetanus toxoid (PTAP) as well as with typhoid-tetanus vaccine (TY-TE) containing lipopolysaccharide (LPS). Both vaccines contained 5.0 Lf (limes flocculans, Ramon) per single dose of tetanus toxoid, purity degree: 1500 Lf/mg protein nitrogen (PN). Tetanus antitoxin titres (TAT) were measured after the first shot, and subsequently before and after booster. Compared to TAT of male animals, significantly lower titres were found in female animals after basic immunisation with PTAP in all the three age groups: 1.03 vs. 0.57, 8.75 vs. 5.64, and 0.27 vs. 0.15 IU (international units, related to the Copenhagen International Standard) per ml (sex-chromosome-dependent differences?), as well as in adult animals immunised with TY-TE, before booster: 0.07 vs. 0.02 IU/ml (hormone-dependent differences?). In the latter case the TAT results after booster were 14.49 vs. 12.89 IU/ml. Thus, the lower female prebooster titres were counterbalanced by a quick and effective increase of titres following booster. These results are in accordance with our previous observations in humans (Réthy and Réthy, 1986). From our observations with tetanus immunisation series on guinea-pigs it can be concluded that TAT may be influenced by the effects of sex chromosomes as well as of sexual hormones. During active anti-tetanus immunisation with LPS-containing vaccine (TY-TE) the lower adult female prebooster titres are presumably counterbalanced by the better functionality of the female immune memory.
Key words: Age, sex, anti-tetanus immunity, LPS, immunomodulation, immune memory
*
Present address and working place: BETHESDA Children’s Hospital of the Reformed Church, H-1147 Budapest, Bethesda u. 3, Hungary+
Corresponding author; E-mail: retlaj@yahoo.com; Fax: +36 (1) 466-4329Acta Veterinaria Hungarica 49 (1), pp. 31–37 (2001)
PHARMACOKINETICS AND BIOAVAILABILITY
OF DOXYCYCLINE IN FASTED
AND NONFASTED BROILER CHICKENS
Department of Pharmacology and Toxicology, Faculty of Veterinary
Science,
Szent István University, H-1400 Budapest, P.O. Box 2, Hungary
(Received July 7, 2000; accepted October 30, 2000)
The pharmacokinetics and the influence of food on the kinetic profile and bioavailability of doxycycline was studied after a single intravenous (i.v.) and oral dose of 10.0 mg/kg body weight in 7-week-old broiler chickens. Following i.v. administration the drug was rapidly distributed in the body with a distribution half-life of 0.21 ± 0.01 h. The elimination half-life of 6.78 ± 0.06 h was relatively long and resulted from both a low total body clearance of 0.139 ± 0.007 L/h·kg and a large volume of distribution of 1.36 ± 0.06 L/kg. After oral administration to fasted chickens, the absorption of doxycycline was quite fast and substantial as shown by the absorption half-life of 0.39 ± 0.03 h, the maximal plasma concentration of 4.47 ± 0.16 µg/mL and the time to reach the Cmax of 1.73 ± 0.06 h. The distribution and the final elimination of the drug were slower than after i.v. administration. The absolute bioavailability was 73.4 ± 2.5%. The presence of food in the intestinal tract reduced and extended the absorption (t1/2a = 1.23 ± 0.21 h; Cmax = 3.07 ± 0.23 µg/mL; tmax = 3.34 ± 0.21 h). The absolute bioavailability was reduced to 61.1% ± 4.4%.
Key words: Doxycycline, pharmacokinetics, bioavailability, chicken, food
*
Present address: Department of Food Hygiene, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary;E-mail: placzay@univet.hu; Fax: +36 (1) 478-4155
Acta Veterinaria Hungarica 49 (1), pp. 39–51 (2001)
MECHANISM OF ACTION OF NORADRENALINE
ON SECRETION OF PROGESTERONE AND OXYTOCIN
BY THE BOVINE CORPUS LUTEUM IN VITRO
Grażyna
Miszkiel and J. Kotwica*Division of Reproductive Endocrinology and Pathophysiology,
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences,
10-718 Olsztyn-Kortowo, Poland
(Received December 7, 1999; accepted October 30, 2000)
The present studies were conducted: (1) to determine which ß-adrenoceptor subtypes are involved in progesterone and oxytocin (OT) secretion, (2) to examine whether noradrenaline (NA) acts directly on the cytochrome P-450scc and 3ß-hydroxysteroid dehydrogenase (3ß-HSD), and (3) to study the effect of prostaglandin F2
a (PGF2a) on NA-stimulated steroidogenesis in luteal cells. The effect of NA on progesterone secretion from luteal slices of heifers on days 8–12 of the oestrous cycle was blocked by both atenolol (ß1-antagonist) and ICI 118.551 hydrochloride (ß2-antagonist). OT secretion was blocked only after treatment with ICI 118.551 hydrochloride (P < 0.05). Dobutamine (10–4–10–6M), a selective ß1 agonist and salbutamol (10–4–10–6M), a selective ß2 agonist, both increased progesterone production (P < 0.01) with an efficiency comparable to that produced by NA (P < 0.01). The increase of OT content in luteal slices was observed only after treatment with salbutamol at the dose of 10–5M (P < 0.01). Dobutamine had no effect on OT production at any dose. A stimulatory effect of NA on cytochrome P-450scc activity (P < 0.05) was demonstrated using 25-hydroxycholesterol as substrate. 3ß-HSD activity also increased following NA (P < 0.01) or pregnenolone (P < 0.05) and in tissue treated with pregnenolone together with NA (P < 0.01). PGF decreased progesterone synthesis (P < 0.05) and 3ß-HSD activity (P < 0.01) in tissue treated with NA. We conclude that NA stimulates progesterone secretion by luteal ß1- and ß2-adrenoceptors, while OT secretion is probably mediated only via the ß2-receptor. NA also increases cytochrome P-450scc and 3ß-HSD activity. PGF inhibits the luteotropic effect of NA on the luteal tissue.Key words: Cattle, corpus luteum, noradrenaline, progesterone, oxytocin
*
Corresponding author; E-mail: janko@pan.olsztyn.pl; Fax: +48 (89) 524-0347Acta Veterinaria Hungarica 49 (1), pp. 53–63 (2001)
PERIPHERAL CIRCULATING INSULIN-LIKE GROWTH
FACTOR-I AND -II IN CATTLE
1
INEP – Institute for the Application of Nuclear Energy, Banatska 31B, 11080 Zemun, Yugoslavia; 2Faculty of Veterinary Medicine, University of Belgrade, Belgrade; 3Institute for Animal Husbandry, Zemun, Yugoslavia; 4Faculty of Veterinary Science, Szent István University, Budapest, Hungary(Received November 11, 1999; accepted October 30, 2000)
Interrelationships between circulating concentrations of the insulin-like growth factors (IGF-I and IGF-II) were investigated in 235 blood samples taken from 145 healthy beef or dairy calves, bulls and cows of different breeds and ages. Autoradiography of Western ligand blots indicated different IGF binding protein (IGFBP) profiles between sera from different categories of cattle. Each IGF radioimmunoassay was validated by determining the effects of IGFBPs, ligand and contraligand, as well as serial dilution and comparison with results obtained after molecular sieve chromatography in acid. In female cattle mean values for IGF-I varied from 5.1 nmol/l in postparturient Holstein cows to 18.5–20.5 nmol/l in growing beef heifers, while mean IGF-II concentrations ranged from 30.0 nmol/l in the cows to 14.7–15.7 nmol/l in the beef heifer calves. In male cattle mean serum IGF-I ranged widely from 8.2 nmol/l in 1-day-old Holstein calves to 67.4 nmol/l in 16-month-old Simmental-type bulls. Mean IGF-II concentrations decreased from 22.9 nmol/l in 1-day-old Holstein bull calves to 11.9 nmol/l in 12-month-old beef bulls. Thus, total molar IGF concentrations were fairly stable in female cattle (24.7–35.1 nmol/l) but extended from 27.3 nmol/l to 81.8 nmol/l in the male cattle. The tendency for a reciprocal relationship between serum concentrations of these growth factors was most obvious in the periparturient cows.
Key words: IGF-I, IGF-II, IGFBPs, radioimmunoassay, cattle
*
E-mail: anikolic@inep.co.yu; Fax: +381 (11) 618-724Acta Veterinaria Hungarica 49 (1), pp. 65–70 (2001)
INSULIN-LIKE GROWTH FACTOR BINDING PROTEINS AND MITOGENIC ACTIVITY OF PARTIALLY FRACTIONATED SHEEP AMNIOTIC FLUID
*, Zuzana Kostecká1, Françoise Cavaille2, M. G. Lacroix31
Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine, 041 81 Košice, Komenského 73, Slovakia; 2INSERM U 142, Hôpital(Received March 22, 2000; accepted May 3, 2000)
Amniotic fluid collected from ewes on various days of gestation was examined for the presence of insulin-like growth factor (IGF) binding proteins. IGF-binding proteins with a molecular mass of 40–45 kDa appeared at day 41 of gestation. The level of these major IGF-binding proteins increased during pregnancy and reached a maximum at day 106. Smaller IGF-binding molecules with an approximate molecular mass of 35 kDa and 25 kDa appeared at day 90, also reaching a concentration peak at day 106. The mitogenic activity of sheep amniotic fluid after chromatography on Sephadex G-50 was separated into two peaks. The peak having lower molecular mass corresponded to an elution profile of 125I-IGF-I. The first peak, having higher molecular mass, was eluted immediately after the void volume of column. Electrophoresis and ligand blotting showed that proteins in the first peak had similar properties as IGF-binding proteins.
Key words: Amniotic fluid, sheep, growth factors, binding proteins
*
E-mail: kostecka@uvm.skActa Veterinaria Hungarica 49 (1), pp. 71–79 (2001)
APOPTOTIC CELL DEATH IN THE PORCINE ENDOMETRIUM
DURING THE OESTROUS CYCLE
1
Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal(Received May 17, 2000; accepted October 30, 2000)
It has been reported that apoptosis plays an essential role in controlling the physiological cell kinetics in the human and rodent endometrium but this type of death has never been studied in the porcine endometrium. The aim of this study was to investigate the apoptotic cell death in the porcine endometrium during the middle (Days 9–11) and late (Day 13) luteal phase, during the luteolysis (Day 15) and early follicular phase (Days 17–19) of the oestrous cycle. Apoptotic cells were identified by in situ DNA 3’-end labelling method. It was revealed that the greatest number of apoptotic cells in the luminal and glandular epithelium was found on Days 17–19 and on Day 15 of the oestrous cycle, respectively. In the stroma, the greatest number of these cells was found on Days 9–11. Our data have shown that in the porcine endometrium, both epithelial and stromal cells undergo apoptosis and that the number of apoptotic cells varies depending on the phase of the oestrous cycle.
Key words: Apoptotic cells, porcine endometrium, TUNEL method
*
E-mail: bwasow@pan.olsztyn.pl; Fax: +48 (89) 524-0347Acta Veterinaria Hungarica 49 (1), pp. 81–86 (2001)
ABSORPTION OF LEUCINE, ALANINE AND LYSINE
FROM THE RUMEN
T. Veresegyházy1*, Hedvig Fébel2 and Ágnes Rimanóczy1
1Department of Physiology and Biochemistry, Faculty of Veterinary
Science,
Szent István University, H-1400 Budapest, P.O. Box 2; 2Department of
Physiology,
Research Institute of Animal Breeding and Nutrition, Herceghalom, Hungary
(Received June 23, 2000; accepted October 30, 2000)
The absorption of three amino acids (leucine, alanine and lysine) from the washed, closed rumen was studied in a short-term (75 min) experiment in situ. The concentration of leucine and alanine did not change in the rumen during the experiment, while that of lysine continuously decreased, and 40% of the total lysine placed in the rumen was absorbed during the experimental period. The rate of absorption decreased in proportion to the fall of amino acid concentration.
Key words: Amino acids, leucine, alanine, lysine, absorption, rumen
*
E-mail: tveres@univet.hu; Fax: +36 (1) 478-4165Acta Veterinaria Hungarica 49 (1), pp. 87–98 (2001)
RADIODIAGNOSTIC EXAMINATION OF THE SWIMBLADDER OF SOME FISH SPECIES
A. Beregi1*, Cs. Székely3, L. Békési2, Judit Szabó1, V. Molnár1 and K. Molnár3
1Department and Clinic of Internal Medicine and 2Department
of Parasitology and
Zoology, Faculty of Veterinary Science, Szent István University, Budapest, H-1400,
P.O. Box 2, Hungary; 3Veterinary Medical Research Institute, Hungarian Academy
of Sciences, Budapest, Hungary
(Received March 16, 2000; accepted October 30, 2000)
Radiodiagnostic methods have not been used previously for studying the anatomy and diseases of the swimbladder of freshwater fish species. In this study, the radiographic anatomy of the swimbladder and species-related differences in swimbladder structure were studied on plain radiographs taken of 12 Hungarian fish species of major economic importance. Changes observed by radiography were also studied by conventional parasitological methods. The radiodiagnostic method reported here appears to be a useful complement to diagnostic examinations that have been based merely on dissection so far. It enables evaluation of the pathological lesions in live condition, without causing damage to the fish.
Key words: Swimbladder, radiodiagnosis, swimbladder changes
*
E-mail: aberegi@ns.univet.hu; Fax: +36 (1) 478-4137Acta Veterinaria Hungarica 49 (1), pp. 99–109 (2001)
PREIMPLANTATION GENETIC DIAGNOSIS IN CATTLE:
A REVIEW
S. Bodó1,2,
*, B. Baranyai2, Elen Gócza2, J. Dohy3 and Merja Markkula11
Agricultural Research Centre of Finland, MTT, FIN-31600 Jokioinen, Finland; 2Agricultural Biotechnology Centre, H-2100 Gödöllő, Hungary;(Received May 8, 2000; accepted October 30, 2000)
article presents the technical requirements for PGD, and shows that this biotechnological method has great economic potential.Preimplantation Genetic Diagnosis (PGD) is reviewed and novel fields where it may be applied are investigated. Technical advances of PGD in cattle embryos have already enabled its integration as a part of the MOET (Multiple Ovulation Embryo Transfer) breeding system. PGD for well-defined selection targets can enhance cattle breeding and embryo trade. It allows embryo selection according to their sex, and it may be used to breed special cow lines, or top bulls, by selecting embryos for valuable production traits using Marker Assisted Selection (MAS). A good allelic profile and/or the insertion of a transgene can be detected by PGD. This review
Key words: Preimplantation genetic diagnosis, embryo, cattle
*
Corresponding author: Agricultural Biotechnology Centre, P.O. Box 411, H-2001 Gödöllő, Hungary; E-mail: bodo@abc.hu; Fax: +36 (28) 430-647Acta Veterinaria Hungarica 49 (1), pp. 111–116 (2001)
THE SURGICAL TECHNIQUE AND THE AGE
OF THE HORSE BOTH INFLUENCE THE OUTCOME
OF MOSAICPLASTY IN A CADAVER EQUINE STIFLE MODEL
G. Bodó1*, A. D. Kaposi2,
L. Hangody3, J. Tóth1, Z. Bakos4, Z. Lukács1
and G. Péntek1
1Department and Clinic of Surgery and Ophthalmology, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary; 2Institute of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary; 3Orthopaedics and Trauma Department, Uzsoki Hospital, Budapest, Hungary; 4Department of Internal Medicine, Faculty of Veterinary Science, Szent István University, Budapest, Hungary
(Received May 10, 2000; accepted October 30, 2000)
Six pieces of grafts, 6.5 mm in diameter, 20 mm in length, were taken from each of 170 cadaver hindlimbs, using the cranial surface of the medial femoral trochlea for harvesting. The age of the horses varied between 4 months and 23 years. 30 limbs under the age of 12 years were selected for transplantation. Three of six grafts were transplanted into the medial femoral condyle using different combinations of tunnel depth and dilation. With ageing, a significant decline in transplantability was detected. In general, mosaicplasty cannot be recommended in horses above 11 years. Based on a previous clinical case (Bodó et al., 2000), a good surface alignment was indeed achieved with a combination of graft length drilling and dilation in most cases. However, the occasional entrapment of cartilage debris under the graft prevented perfect alignment in the present cadaver study in 27% of the grafts transplanted in this manner. Since the protrusion of grafts never exceeded 1.5 mm, we conclude that drilling 3–5 mm deeper than graft length with graft length deep dilation can avoid disadvantageous protrusion of the transplanted hyaline cartilage caps, achieving bone decompression at the same time.
Key words: Horses, autograft transplantation, mosaicplasty, stifle
*
E-mail: gbodo@univet.hu; Fax: +36 (1) 478-4196Acta Veterinaria Hungarica 49 (1), pp. 117–120 (2001)
PATHOGENICITY OF A BOVINE VIRAL DIARRHOEA VIRUS STRAIN IN PREGNANT SOWS: SHORT COMMUNICATION
*, P. Soós2, L. Kucsera1, R. Glávits3 and V. Pálfi31
State Control Institute for Veterinary Biologicals, Drugs and Feeds, H-1415 Budapest 10, P.O. Box 318, Hungary; 2Department of Microbiology and Infectious Diseases, University of Veterinary Science, Budapest, Hungary; 3Central Veterinary Institute, Budapest, Hungary(Received February 25, 2000; accepted May 3, 2000)
The biological properties of bovine viral diarrhoea virus (BVDV) strain Oregon C24V were studied after intranasal and subcutaneous infection of pregnant sows. This virus strain is widely used in Hungary for immunising cattle against bovine viral diarrhoea (BVD). Based upon the results of the clinical, gross pathological, histopathological and virological examinations it can be established that the given strain caused asymptomatic infection and serological conversion in sows that were in the second third of gestation. The virus caused clinically apparent disease in some of the piglets born at term, which indicates that it had crossed the placenta. More than half (57%) of the live-born piglets died within 60 days of birth. The sows and their progeny did not shed the virus. BVDV infection has great differential diagnostic importance in pigs, as classical swine fever (CSF) virus strains of reduced virulence cause similar clinical symptoms and gross and histopathological changes.
Key words: Pestivirus, classical swine fever virus, bovine viral diarrhoea virus, pregnant sow, fetopathic effect
*
E-mail: kulcsar@oai.hu; Fax: +36 (1) 262-2839Acta Veterinaria Hungarica 49 (1), pp. 121–124 (2001)
BOOK REVIEWS
Martin Kessler (ed.): Kleintieronkologie. Diagnose und Therapie von Tumorerkrankungen bei Hunden und Katzen. With the contribution of N. Birnbaum, D. v. Bomhard, M. Brearley, F. Gaschen, U. Gränitz, A. S. Hammer, J. Hirschberger, B. Kaser-Hotz, M. Link, W. Misdorp, L. Moe, H. Müller, M.-L. Nagel, R. Neiger, R. Nickel, S. Pfleghaar, C. Reusch, D. Ruslander, G. Rutteman, J. Siebert, K. Simpson, D. Simon, D. Smeak, N. Sutter-Steinmann, M. Tassani-Prell, E. Teske, and T. Vahlenkamp. Parey Buchverlag, Berlin, 2000. 566 pages, 449 figures and 167 tables. Price: DM 228. ISBN 3-8263-3236-9.
Walter Baumgartner (ed.): Klinische Propädeutik der inneren Krankheiten und Hautkrankheiten der Haus- und Heimtiere 4., vollständig neubearbeitete und erweiterte Auflage. With the contribution of Cornelia J. Ketz Riley, Gerd Schlerka, Maximilian Schuh, Gerald Schusser, Alexandra Scope, Johann Thalhammer, Andrea Tipold and Lore Vasicek. Parey Buchverlag, Berlin, 2000. 482 pages, 103 figures and 35 tables. Price: DM 86. ISBN 3–8263–3238–5.
Michel Rommel, Johannes Eckert, Erich Kutzer, Wolfgang Körting and Thomas Schnieder: Veterinary Parasitology (Veterinärmedizinische Parasitologie). Fifth Edition, Parey Buchverlag, Berlin, 2000. 915 pages, 332 figures, 100 tables, hardcover. Price: DM 228. ISBN 3-8263-3178-8.